Postdoctoral Researcher (Biochemistry, On-site)

Job summary

A highly motivated Postdoctoral Researcher is sought to join a project focused on single-cell proteomics and mass spectrometry, specifically targeting transcription factors in human blood stem cells. This role involves developing innovative workflows to detect MiTag-derived peptides alongside global proteomes, contributing to a groundbreaking interdisciplinary project.

Qualifications

• PhD in biochemistry, (bio)analytical chemistry, cell biology, or a related field.
• Extensive hands-on experience with LC-MS/MS-based proteomics, with at least 2–3 years of operation of high-end instruments (preferably Orbitrap series).
• Strong background in DIA-based proteomics workflows, including hybrid targeted/untargeted acquisition strategies.
• Demonstrated experience in single-cell proteomics, including low-input sample preparation and liquid handling automation.
• Proficiency in computational proteomics data analysis and downstream analysis in R or Python.
• Proven ability to conduct independent research from conception to publication.
• Excellent written and oral communication skills in English.

Responsibilities

• Design, optimize, and deploy scp-MS workflows for detecting MiTag peptides and global proteomes in primary human hematopoietic cells.
• Develop DIA-based scp-MS acquisition methods on the Orbitrap Astral.
• Optimize protocols for intracellular MiTag staining compatible with high-sensitivity scp-MS.
• Benchmark MiTag performance against orthogonal assays.
• Collaborate with other labs to refine reagents and transfer workflows to primary samples.
• Publish results in leading journals and present at international conferences.
• Mentor junior lab members and contribute to a collaborative lab culture.

Skills

• Experience with targeted proteomics approaches and FACS-based cell sorting is desirable.
• Familiarity with mammalian cell culture, particularly primary hematopoietic cells.
• Ability to analyze and interpret larger single-cell datasets.

Education

• A formal qualification of a PhD degree (or equivalent) is required.

Tools

• Experience with DIA-NN, MSFragger, Spectronaut, or similar tools for data analysis.