PhD Researcher in Microbial Physiology

Job summary

This position involves investigating the physiological limits of microbial acetate assimilation through external electron supply. The research aims to enhance the efficiency of acetate utilization in microbial cells, contributing to sustainable bioproduction.

Qualifications

• A two-year master's degree (120 ECTS points) or equivalent.
• MSc in microbiology, biochemistry, microbial physiology, or a related field.
• Strong foundation in microbial physiology and intermediary metabolism.
• Hands-on experience with microbial cultivation, including anaerobic or microaerobic techniques.
• Experience with analytical methods for metabolite and substrate quantification (e.g., GC-FID, LC-MS, ¹³C-labelling).
• Familiarity with molecular biology and basic genetic engineering in bacteria.
• Curiosity, scientific rigor, and enthusiasm for fundamental physiological questions.
• Collaborative mindset and excellent communication skills in English.

Responsibilities

• Establish and characterize stable acetate–C1 co-utilization phenotypes using adaptive laboratory evolution (ALE) and/or targeted metabolic engineering.
• Quantify carbon yields and biomass during co-utilization and validate stoichiometric predictions of carbon retention across different acetate assimilation pathways.
• Map physiological trade-offs and substrate partitioning across acetate:C1 ratios using high-throughput cultivation systems, ¹³C-labelling, and metabolite quantification.
• Investigate how the redox entry point of C1 oxidation controls carbon assimilation flux and retention efficiency.
• Apply transcriptomics, proteomics, and metabolomics to identify regulatory and metabolic adaptations enabling efficient redox balancing.
• Disseminate results through peer-reviewed publications and presentations at international conferences.

Education

• A master's degree in a relevant field is required.

Tools

• Experience with microbial cultivation techniques and analytical methods for metabolite quantification.